rabbit polyclonal antibody against abca1  (Novus Biologicals)

Journal: Biomedicines

Article Title: Small GTPase ARL4C Associated with Various Cancers Affects Microtubule Nucleation.

doi: 10.3390/biomedicines12122872

Figure Lengend Snippet: Figure 2. Treatment using LXR/RXR ligands does not lead to an increase in the endogenous ABCA1 or ARL4C level in many cell lines: (A) Titration of BS-C-1 lysate demonstrates that the ratio of levels of ARL4C in Vero and BS-C-1 cells is 1:4.5. (B) The level of ARL4C is not increased in both Vero and BS-C-1 cells even after 7 days of treatment with T0901317 and bexarotene. (C) LXR/RXR-dependent pathway is activated in HeLa cells and weakly activated in MCF7 cells after 48h exposure to 2.5 µM T0901317/bexarotene; however, not in Vero cells even after prolonged treatment. (D) Neither ABCA1 nor ARL4C levels increase after treatment of COS7 and U2OS cells with RXR/LXR ligands. Long-term treatment of the MCF7 cells results in only a slight increase in the ARL4C level.

Article Snippet: Then, the membrane was blocked with 5% skimmed milk in TBST for 1 h. Rabbit polyclonal antibody against ARL4C (Novus Biologicals, Centennial, CO, USA), mouse monoclonal antibody against GAPDH (ThermoFisher Scientific, Waltham, MA, USA), rabbit polyclonal antibody against ABCA1 (Novus Biologicals, Centennial, CO, USA), mouse monoclonal antibody against α-tubulin, and clone DM1A (Santa Cruz Biotechnology, Dallas, TX, USA) were used as primary antibodies.

Techniques: Titration

Journal: Acta Pharmaceutica Sinica. B

Article Title: PPAR α affects hepatic lipid homeostasis by perturbing necroptosis signals in the intestinal epithelium

doi: 10.1016/j.apsb.2024.08.021

Figure Lengend Snippet: Pparα deficiency in intestinal epithelium increases the translocation of gut-derived antigens into the liver. (A) Intestinal permeability assessment (FITC-dextran, 4 kD) in 8-week-old mice ( n = 10). (B) Relative mRNA levels of Zo-1 and Cldn8 in the ileum from 8-week-old mice ( n = 5). (C) The relative proportion of bacterial species in the cecum content by 16S rRNA gene sequencing ( n = 6). (D) Bugbase phenotypic prediction of gut microbiota in 8-week-old mice ( n = 6). (E) The mRNA and protein levels of PV1 in the ileum of 8-week-old mice ( n = 5). (F) Transmission electron microscopy images of the diaphragm (red star) in the capillaries from ileum sections in 24-week-old mice ( n = 3). (G) Representative images of fluorescence microscopy and transmission electron in 8-week-old mice treated with FITC-LPS (green) or Au-LPS ( n = 3–5). (H) Portal HDL-C levels in 8-week-old mice ( n = 10). (I) The protein levels of APOA1 and ABCA1 in the ileum of 8-week-old mice ( n = 5). (J) Relative mRNA levels of Apoa1 , Pon1 , and Pon3 in the ileum of 8-week-old mice ( n = 5). (K) Serum APOA1 levels in 8-, 16- and 32-week-old mice ( n = 8–10). (L) Serum APOA1 levels in 8-week-old mice exposed to HFCS for 14 days ( n = 8). (M) Serum APOA1 levels in 16-week-old Pparα Δhep mice ( n = 10). (N) Schematic representation of a cocktail of broad-spectrum antibiotics (Abx) experimental design. (O) Representative images stained with H&E and Oil Red O, and immunofluorescent staining for F4/80 (red) in the liver from 8-week-old mice treated with Abx ( n = 5). (P) Triglyceride in serum and liver treated with Abx ( n = 10). (Q) Relative mRNA levels of genes related to triglyceride accumulation in the liver from 8-week-old mice treated with Abx ( n = 5). (R) Hepatic levels of cytokines from 8-week-old mice treated with Abx ( n = 5). (S) Protein levels of F4/80 in the liver of 8-week-old mice treated with Abx ( n = 3). (T) Relative mRNA levels of F4/80, Clec4f , and Cd14 in the liver of 8-week-old mice treated with Abx ( n = 5). LD: lipid droplet; M: mitochondria; FITC-LPS: fluorescein isothiocyanate (FITC)-LPS; Au-LPS: LPS-gold-complexes. Data are shown as the mean ± SD. An unpaired two-tailed Student's t -test; ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: These samples were incubated overnight with a polyclonal rabbit anti-mouse F4/80 (1:1000, #A1256, ABclonal), polyclonal rabbit anti-mouse APOA1 (1:2000, #A14211, ABclonal), polyclonal rabbit anti-mouse ABCA1 (1:1000, #NB400-105, Novus Biological, Centennial), monoclonal rabbit anti-mouse CD14 (1:1000, #A19011, ABclonal), or polyclonal rabbit anti-mouse PV1 (1:2000, #A15906, ABclonal).

Techniques: Translocation Assay, Derivative Assay, Permeability, Sequencing, Transmission Assay, Electron Microscopy, Fluorescence, Microscopy, Staining, Two Tailed Test

Journal: iScience

Article Title: Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

doi: 10.1016/j.isci.2024.110144

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-ABCA1 , Cell Signaling Technology , Cat# 96292.

Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software